Current and future application of stem cell research
Stem Cells Key Words: Embryonic stem cells, Adult stem cells, iPS cells, self-renewal, differentiation, pluripotent, multipotent, Inner cell mass, Nuclear transfer (Therapeutic cloning), Feeder cells, LIF, embryoid body. What are stem cells? Cells that are able to renew themselves (Self-renewal) indefinitely, while producing cell progeny that mature into specialized
cells (Differentiation). Throughout our lives, stem cells in our body regenerate cells to renew damaged tissues such as skin and blood. Found throughout your life in tissues, blood, bone marrow and adipose. Types of stem cells Embryonic stem cells- can generate all
cell types (Pluripotent vs. Totipotent) Adult stem cells (also called somatic or tissue-specific stem cells)- can generate cell types within a specific tissue or organ (Multipotent) Induced pluripotent stem cells (iPS)engineered from specialized cells (Pluripotent).
Embryonic stem cells (ESC) Embryonic stem cells are obtained from the inner cell mass of blastocysts Blastocyst is formed early in the development (5 days after fertilization). Inner cell mass
Early Development: Cells are Segregated Into 4 Different Cell Types: Ectoderm, Endoderm, Mesoderm, and Primordial Germ Cells [Image taken from Gilberts Developmental Biology, 8th edition, Sinauer]. Differentiation The process by which cells develop into specialized cells with specific
functions and structures. Differentiation into specialized cells Totipotent Pluripotent Multipoten t Ectodermal
cell Unipotent brain skin Zygote
bone marrow ES cell Mesoderm al cell Image from Stanford stem cell
heart Primitiv Differentiat ed7 e cells progenit
Applications for stem cells Potential to treat or cure diseases by tissue replacement A model to study early human development and developmental disorders A model to study gene regulation and development Drug discovery and toxicology studies Used to supply cells for the repair of damaged or diseased organs Examples:
Bone marrow transplantation Skin replacement Blood disorder treatments (Dry) Macular degeneration www.isscr.org Sources of Embryonic stem cells Blastocysts created in culture for IVF (in vitro fertilization) that are not implanted
into uterus Therapeutic cloning Sources of ES cells Sources of ES cells In vitro fertilization (IVF): Isolate sperm and egg from male and female,
mix together fertilized egg (zygote) Cultured for 2-5 days blastocysts Blastocysts implanted into uterus Many are not implanted into uterus, and can be used to make ES cells
Isolation of embryonic stem cells from the blastocyst. Therapeutic or patient-specific cloning (Nuclear transfer) Used to avoid immune rejection from the patient.
The blastocyst that is generated this way is not implanted in the uterus (reproductive cloning) and therefore it does not develop into an embryo. Has been successful in mice but difficult in humans. Therapeutic Cloning nucleus of an egg is replaced by the nucleus of the patients
cell Disadvantages of embryonic stem cells Difficult to induce certain differentiation pathways Can trigger immune response in the recipient individual (unless therapeutic cloning is used to generate stem cells) Could become cancerous (teratoma tumors) Controversial ethical and political issues
Repaired heart Adult stem cells from a healthy mouse are injected into damaged heart of another mouse.
Culturing of embryonic stem cells The inner cell mass of the blastocyst is separated from the trophoectoderm that surrounds it. The cells are cultured in a culture dish with or without feeder cells. Feeder Cells
Feeder cells are non-invading cells, usually mouse embryonic fibroblasts that have been inactivated so they do not divide. Feeder cells provide various growth factors and contact embryonic stem cells. Feeders help the ESCs to maintain their pluripotency. Since feeder cells can potentially contaminate the stem cells it is preferred to grow stem cells
without feeders. Mouse ES cells growing on feeders. ES Cell Clusters Feeders (Mouse Embryonic Fibroblasts) Human embryonic stem cell colony growing on feeders.
Stem cells in culture tend to aggregate to form colonies. In some colonies cells may differentiate spontaneously. To prevent differentiation, cells need to be passaged (subcultured) frequently. The colonies are removed and dispersed into single cells and cultured again.
Leukemia Inhibitory Factor (LIF) Mouse embryonic stem cells can keep their pluripotency without feeder cells if LIF is added to the media. LIF binds LIF-receptors on the surface of mouse ES cells and triggers activation of the transcription factors that are necessary for continued proliferation. LIF is added to the media to inhibit differentiation of the cells and to maintain their self-renewal property (Pluripotency). To trigger differentiation, LIF is removed from the culture.
Human ESCs are not responsive to LIF. Human ESCs can grow in undifferentiated state without feeders if the media has been conditioned with human or mouse cells before use. Feeder Independent mESC cultured in the presence of LIF (Leukemia Inhibitory Factor) Differentiation Method ES cells differentiate spontaneously into all three forms
of cells (ectoderm, mesoderm and endoderm) if the right conditions are provided To trigger differentiation, ES cells are grown in the absence of LIF and on uncoated plates to prevent adhesion to the plates. The cells form aggregates (spheres) called embryoid bodies. Differentiation initiates spontaneously upon aggregation of cells.
Under appropriate culturing conditions ESCs can be directed to differentiate into specific cell types. Pluripotent ESCs Week 1- Floating EBs ESCs in suspension
- LIF + RA Week 2-Differentiated neurons Differentiated neurons MAP2 protein in red
Whats Been Done to This Point mESC (E14) grown using enriched pluripotent media (PPM) DMEM / high glucose
L-glutamine Sodium pyruvate Non-essential amino acids 2-mercaptoethanol Luekemia Inhibitor Factor (LIF) T-25 tissue culture vessels to adhere Whats Been Done to This Point
Day 1 Monday, 7/8 Plated as suspension in low-binding plates => embryoid bodies (Ebs) in differentiation media Feed at day 3 Begin Directed Differentiation day 5 of EB culture - transfer Ebs Half in Differentiation Media => spontaneous Half in Differentiation Media w/ 5 uM Retinoic
acid => directed to neurons What You Will Do Today Treat 24-well TC plate with 0.1% gelatin Harvest Ebs 10 miutes in centrifuge tube (incubator) Aspirate and replace media Half in Differentiation Media => spontaneous Half in Differentiation Media w/ 5 uM Retinoic
acid => directed to neurons What You Will Do Today Dispense into treated wells at 5 to 10 Ebs per well Issues Aseptic transfers Treat Ebs gently, especially when resuspending Will the Ebs cooperate => cardiomyocytes and
neurons Differentiated neurons Cardiomyocytes Glossary Stem cells- Cells that are able to renew themselves indefinitely, while producing cell progeny that mature into specialized cells. (Self renewal and differentiation)
Self-renewal- The ability of cells to divide and produce more of themselves Differentiation- The process of development with an increase in specialization Blastocyst- A very early embryo. Contains the inner cell mass which forms the embryo and trophoblast that forms the placenta. Therapeutic cloning- The use of cloning by nuclear transfer to produce an embryo that will provide embryonic stem cells to be used in therapy. Multipotent stem cells- Stem cells whose progeny are able to mature into multiple differentiated cells, but all within a particular tissue. Pluripotent stem cells- Stem cells that can become all cell types. Except for
trophoblast. Totipotent cells- Zygote and the first cells that are produced in the days of development before blastocyst formation . These Cells can become all cell types. Embryoid body- Spheroid colonies seen in culture produced by the growth of embryonic stem cells in suspension. Sadava et al 2007 www.isscr.org
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